Purification of black chokeberry procyanidins and determination of epicatechin and procyanidins B2 by HPLC

The adsorption and desorption properties of procyanidins from black chokeberry with AB-8 macroporous resins was studied. And the HPLC method was used to determine the content of epicatechin and procyanidins B₂ in procyanidins purification.The results showed that, procyanidins concentration of the sample solution was 4.0 mg/m L, adsorption flow rate was 2 BV/h, the desorption concentration of ethanol was 50%. Under the proposed conditions, the purity of procyanidins content from black chokeberry was 14.29%. Chromatographic separation was performed on the Aglient Eclipse XDB-C18, 4.6 mm ×250 mm. The mobile phase was methanol ( A) -water ( B) with the gradient elution ( 0 ¹0 min, 10% A-30% A; 10 ²0 min, 30% A-70% A; 20 ~ 30 min, 70% A-100% A) . The flow rate was 1.0 m L/min and the detective wavelength was set at280 nm. The linear ranges of epicatechin and procyanidins B2 concentration were 0.025⁰.2 mg/m L.The sample recoveries rate of the two components were 105.5% and 99.8% respectively. The content of epicatechin and procyanidins B₂ in black chokeberry procyanidins purification were 14.26 mg/g and 22.45 mg/g, respectively.