Optimization of enzymatic hydrolysis conditions for duck gizzard antioxidant peptide preparation by response surface methodology

Based on the single factor experiment, the response surface method was used to optimize the preparation process of duck gizzard antioxidant peptide.In the pre-screening of the best protein for enzymatic hydrolysis of duck gizzard, papain was obtained as the best enzyme for enzymatic hydrolysis of duck gizzard protein.The optimum conditions were as follows: the time of enzymolysis was 4.0 h, the amount of enzyme was 0.5%, the enzymolysis temperature was 55 ℃, and the ratio of solid to liquid was 1∶ 3 g/m L.Under these conditions, the hydrolyzate degree of hydrolyzed duck gizzard protein was 28.30% and the DPPH·clearance rate was 75.10%.The results showed that the duck gizzard antioxidant peptide preparation process was feasible, there was no correlation between the hydrolysis degree of duck gizzard and DPPH· scavenging ability.