Cloning and sequence analysis of a resveratrol synthase gene from Vitis vinifera

A complete c DNA sequence of resveratrol synthase gene was obtained from Vitis vinifera total RNA by RT-PCR method, named RS.The results of multiple sequence alignment showed that RS was of 1179 bp with 94% ~ 99% nucleic acid sequence similarity and 96% ~ 99% amino acid sequence similarity to the reported Vitis vinifera resveratrol synthase, respectively.Domain analysis results revealed that the characteristics sequences GVLFGPGLT and the active site sequences GCYAGGTVLR of stilbene synthase family were found in cloned RS amino acids sequence.The predicted molecular weight was of 42.78 k Da and the theoretical isoelectric point was of 6.57.The calculated instability parameter was of 35.92, which indicated that this protein was stable in classification.The secondary structure of resveratrol synthase mainly contained α-helix, random curling and β-sheet, which content was of 44.13%, 26.53% and 17.66%, respectively.