Determination of lactic acid and acetic acid content in biological selenium vinegar and vinegar by HPLC method

WANG Xing-hua; LIU Ju; XIE Yu-tao; SONG Jia; ZHANG Fan; QIAO Shen

doi:10.13386/j.issn1002-0306.2017.16.047

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Science and Technology of Food Industry > 2017 > (16): 247-252. > DOI: 10.13386/j.issn1002-0306.2017.16.047

WANG Xing-hua, LIU Ju, XIE Yu-tao, SONG Jia, ZHANG Fan, QIAO Shen. Determination of lactic acid and acetic acid content in biological selenium vinegar and vinegar by HPLC method[J]. Science and Technology of Food Industry, 2017, (16): 247-252. DOI: 10.13386/j.issn1002-0306.2017.16.047

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Determination of lactic acid and acetic acid content in biological selenium vinegar and vinegar by HPLC method

School of Life Science, Shanxi University

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Received Date: February 28, 2017

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Determination of lactic acid and acetic acid of biological selenium vinegar and vinegar by using HPLC method were studied.C₁₈ as chromatographic analysis column, detection wavelength was 215 nm, the mobile phase was 2.5% NH4H2PO4 buffer, sample dilution was 30 times, the flow rate was 1.0 min/m L, the column temperature was 29 ℃, the best p H of the biological selenium vinegar was 2.5, while the best p H of the vinegar was 2.7, which could better separation and determination of lactic acid and acetic acid of biological selenium vinegar and vinegar, all testing completed within 4 min.When other analysis conditions were certain, p H was 2.5, the regression equation of acetic acid and lactic acid standard were y = 484.23x-81.373, R²= 0.9992, y = 441.37x-68.321, R²= 0.9979, respectively.When p H was 2.5, the regression equations of acetic acid and lactic acid standard were y = 484.61 x + 72.201, R²= 0.9993, y = 389.82 x + 30.432, R²= 0.9991, respectively. The relative standard deviation of this method was 0.00% ¹.07%, standard addition recoveries of biological selenium vinegar lactic acid and acetic acid standard were 100.00% ¹10.00%, 99.00% ¹00.83% respectively, standard addition recoveries of vinegar lactic acid and acetic acid were 100.00% ~ 101.00%, 100.00% ~ 110.00% respectively. The method has good repeatability, accuracy and precision, short detection time, therefore it could be applied in biological selenium vinegar and vinegar.
- HPLC method,
- biological selenium vinegar,
- vinegar,
- lactic acid,
- acetic acid

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Determination of lactic acid and acetic acid content in biological selenium vinegar and vinegar by HPLC method