Evaluation of antioxidant activity in vitro of anthocyanins and polysaccharides in the seed coat of Ampelopsis grossedentata

To research antioxidant activity in vitro of anthocyanins and polysaccharides in the seed coat of Ampelopsis grossedentata.The contents of anthocyanins and polysaccharides were determined by p H differential spectrophotometry and UV visible spectrophotometry respectively.To investigate the scavenging ability of anthocyanins and polysaccharides to ·OH, O-2·and DPPH·.It turned out that the order of anthocyanins and polysaccharides on the value of ICO₂^- of DPPH·was polysaccharides ( 0.06 mg/m L) > anthocyanins ( 0.09 mg/m L) > VC ( 0.15 mg/m L) .The correlation coefficent of the values of ICO₂^- of·OH, O-2·and DPPH· and the content of anthocyanins were-0.987 ( p < 0.05) , -0.958 ( p < 0.05) and-0.995 ( p < 0.01) , but were not significantly correlated with the content of polysaccharides Anthocyanins and polysaccharides had better scavenging ability of DPPH·, especially the ability of polysaccharide.It was significant correlation between the content of anthocyanin and its scavenging activity of DPPH radical ( p < 0.01) .