Effect of ultra-high pressure on the physicochemical properties of actomyosin from bighead crap (Arstichthys nobilis)

LIU Chun-hua; LIANG Yan; ZHOU Ai-mei; XIAO Su-yao; LIU Xin; CAO Yong

doi:10.13386/j.issn1002-0306.2017.16.007

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Science and Technology of Food Industry > 2017 > (16): 29-34. > DOI: 10.13386/j.issn1002-0306.2017.16.007

LIU Chun-hua, LIANG Yan, ZHOU Ai-mei, XIAO Su-yao, LIU Xin, CAO Yong. Effect of ultra-high pressure on the physicochemical properties of actomyosin from bighead crap (Arstichthys nobilis)[J]. Science and Technology of Food Industry, 2017, (16): 29-34. DOI: 10.13386/j.issn1002-0306.2017.16.007

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Effect of ultra-high pressure on the physicochemical properties of actomyosin from bighead crap (Arstichthys nobilis)

College of Food Science, South China Agricultural University
Guangdong Province Engineering Research Center for Bioactive Natural Products
Pro-Health ( China) Co., Ltd.

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Received Date: February 22, 2017

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Abstract

Abstract

Changes of physicochemical properties in natural actomyosin from Arstichthys nobilis induced by ultra-high hydrostatic pressure ( 100, 300, 500 MPa for 15, 30, 45 min) were investigated. The results revealed that the solubility of actomyosin decreased while the turbidity increased with the increase of pressure and time, indicating the accumulation of protein aggregates and denaturation.SDS-PAGE showed that ultra-high pressure induced the cross-linking and accumulation of actomyosin to form macromolecular substances.Ca²⁺-ATPase activity of actomyosin treated by ultra-high pressure was lost, suggesting the denaturation of actomyosin.Surface hydrophobicity of actomyosin increased when the pressure and pressurization time increased, indicating that the exposed hydrophobic residues increased upon application of high pressure. The total sulfhydryl content of actomyosin decreased while the disulfide bond content increased with the increase of pressure, which confirmed that the sulfhydryl group of actomyosin underwent oxidation to form disulfide bonds. These variations in the physicochemical properties demonstrate that the conformation of actomyosin extracted from Arstichthys nobilis changes after ultra-high pressure processing.
- ultra-high pressure,
- Arstichthys nobilis,
- actomyosin,
- physicochemical properties

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