Optimization concentrate process of shikimic acid from Illicium verum Hook.F.by ultrafiltration-nanofiltration coupling technology

The concentrate process of shikimic acid from Illicium verum Hook. f. were investigated by using ultrafiltration-nanofiltration coupling technology.Based on the single factor experimental analysis and pretreated with ultrafiltration, through the response surface methodology, concentrate condition was optimized by investigating the effect of molecular weight cut-off, concentration, p H and their interaction on the rejection of shikimic acid and the process of quadric polynomial mathematics model was established. The results showed that the rejection of the total protein was 96.88% with 50 k Da ulrafiltration membrane, the regression equation fit well with experimental data and the interaction effect of molecular weight cut-off and concentration was highly significant ( p < 0.000 1) .The optimal concentrate conditions were as follows: molecular weight cut-off100 Da, p H6.20, concentration 10.40 μg/m L.Under these conditions, the rejection of shikimic acid from Illicium verum Hook.f.was ( 96.08 ± 2.02) % closing to predicted rejection 95.00%.