Response surface optimization and purification technology and antioxidant activity of total flavonoids from Kushui rose oil distillation waste

Objective: To investigate response surface optimization and purification technology and antioxidant activity of total flavonoids from Kushui rose oil distillation waste, and provide the basis for the recovery of total flavonoids from Kushui rose oil distillation waste. Methods: Studying on D101 resin adsorption and desorption ability for flavonoids compounds by static experiment, then based on dynamic experiment of single factors, the optimum purification process parameters of Kushui rose oil distillation waste by response surface method.On this basis, with vitamin C ( VC) as the control, study the scavenging ability of total flavonoids purified on ABTS, DPPH, and OH free radical. Results: The static adsorption capacity was 1.83 mg/g, the adsorption rate was 86.10%, the elution rate of 88.07%, which indicated that D101 resin had better adsorption and desorption ability for flavonoids compounds of Kushui rose oil distillation waste. And the optimum dynamic adsorption process parameter were the sample solution p H2.12, sample concentration 10.4 mg/L, sample flow rate 1.72 m L/min, the adsorption rate was95.06%, elution with 60 m L absolute alcohol solution, the desorption rate reached 98.30%, the purity of total flavonoids was up to 5.651%.The IC50 value of total flavonoids purified on ABTS, DPPH, OH free radical were 0.03, 0.1, 8 μg/m L, the maximum scavenging rate were 98.96%, 89.76%, 65.45%, we can see that the total flavonoids from waste has better antioxidant activity.