Biological characteristics and exoenzymes analysis of a mutated strain Bacillus amyloliquefaciens NCU116-1

The biological characteristics and culture characteristics of Bacillus amyloliquefaciens NCU116-1 were studied.Nation-standard method and carboxymethyl cellulose saccharification method were used to analyze the enzymes production fastigium and their properties.The result showed that the optimal fermentation time of amylase, amylase and cellulase was 40 h.The optimum fermentation time of the neutral protease was 44 h. The optimum fermentation time of pectinase was 42 h, The neutral protease had the maximum activity at 50 ℃.Mn2 +was an activator of neutral protease.Its optimum temperature and p H value were 40~45 ℃ and 8 respectively.The glucoamylase had the maximum activity at 35 ℃, and was activated by Ca2 +, Fe3 +and Mn2 +.Its optimum temperatures and p H value were 35~40 ℃ and 8.0 respectively.The pectase had the maximum activity at40 ℃, and was activated by Mn2 +and Ca2 +. Its optimum temperatures and p H value were 35 ~ 40 ℃ and 6.0 respectively. The cellulase had the maximum activity at 50 ℃, and was activated by Ca2 +and Cu2 +.Its optimum temperatures and p H value were40~45 ℃ and 7.0 respectively.The extracellular enzymes produced by the strain meet the requirements of aqueous enzymatic method and this paper lays a theoretical foundation for the application of this kind of strain.