Determination of nine phenolic components in the leaves of Chinese hawthorn by HPLC

Nine phenolic components in the leaves of Chinese hawthorn were determined by RP-HPLC simultaneously. The results indicated that the linearity ranges of nine compounds were between 2 ~ 500 μg/m L and the correlation coefficient ( r) were between 0.9995~0.9999.The recovery was 93.7% ~110.2%.The relative standard deviations were between 0.69% ~4.58%.The average content of nine phenolic components from low to high were isoquercitrin, hyperoside, procyanidin C1, procyanidins D1, epicatechin, proanthocyanidin B2, chlorogenic acid, eucomic acids, vitexin-2″-O-rhamnoside. Among them, the content of flavonoids and phenolic acids was higher than 15 mg/g D.W and the content of proanthocyanidins was up to 6 mg/g D.W.The method of HPLC was simple and accurate.