Optimization of the extraction of glycoprotein from bird's nest by response surface methodology

In this paper, glycoprotein from bird's nest was extracted by the hot water extraction.The effects of extraction time, material to liquid ratio and extraction times on the extraction rate of glycoprotein were studied, and the response surface analysis was used to optimize the extraction conditions of glycoprotein from bird's nest.The optimal condition of glycoprotein extraction was obtained with 2.6 hours of extraction time, 1 : 47 of material to liquid ratio and extracting for 2 times. The extracts were concentrated and precipitated. Finally, the crude glycoprotein was achieved by dialysis and lyophilization with the yield of 3.01%.In addition, the protein content and polysaccharide content in the crude glycoprotein prepared by this method were 59.06% and 19.2%, respectively.