Ultrasonic-assisted enzymatic method extraction of polysaccharide from Armillaria mellea

Objective: In order to study the extraction technology of polysaccharide from Armillaria mellea, based on the polysaccharide yield, polysaccharide was extracted by ultrasonic-assisted enzymatic ( cellulase, papain) method. Method: This paper studied the optimal extraction conditions by single factor experiment, such as enzymolysis temperature, ultrasonic power, ultrasonic time, liquid material ratio, enzymolysis time, ratio of compound enzyme, enzyme concentration.The optimal conditions were obtained by response surface methodology on the basis of single factor experiment. Result: By single factor experiment, enzymolysis temperature 50 ℃, ultrasonic power 360 W, ultrasonic time 20 min were determined.The results of response surface methodology showed that the optimal extraction conditions were enzyme concentration 1.9%, ratio of compound enzyme 2 : 1, enzymolysis time 138 min, liquid material ratio 30: 1 ( mL/g) .Conclusion: Under the optimal extraction conditions, the yield of polysaccharide reached 40.56%.