Intervention effects of glucosamine,drynaria rhizome and astaxanthin on acute osteoarthritis in rats

Objective: To investigate and compare the individual and combined effects of glucosamine,astaxanthin,drynaria rhizome on acute osteoarthritis in rats,so as to provide theoretical basis for the development of functional foods with preventive and improvement effects on osteoarthritis.Methods: 120 male SD rats were randomly divided into 10 groups: the control group,model group,positive group and 7 treatment groups( GLU,DR,AST,GLU + DR,GLU + AST,DR + AST,GLU + DR + AST group). The control and model group were orally given distilled water( 10 m L / kg),the positive group received diclofenac sodium( 2 mg / kg),the gavage dose of GLU,DR and AST was respectively 1000,250,120 mg / kg.The arthritis was induced by injecting the articular cavity with kaolin and carrageen- λ,the degree of joint swelling was measured on 1 th,3 th,5 th,and 7 th days after modeling.The knee joints were subjected to prepare paraffin tissue sections to observe the pathological changes of articular cartilage and to evaluate the reduction of proteoglycan.The contents of TNF- α and IL-1β in synovium were detected with ELISA kits.Results: Compared with model group,the swelling of the knee joint showed different degrees of reduction in each treatment group,the changes were more significant in GLU,DR and GLU + DR group( p < 0.01).The histopathological score and the contents of TNF- α and IL-1β in synovium were decreased in different degrees in each treatment group,the GLU+ DR,GLU + DR + AST and GLU DR group displayed a more significant reduction( p < 0.01). Conclusion: GLU and DR had preventive and protective effects on acute osteoarthritis in rats,and the combined effect was better. While AST had relativelyminimum effect.