The rapid mutant strains of high tannase yield obtained by atmospheric and room temperature plasmas

Atmospheric and room temperature plasmas( ARTP) was used to induce Aspergillus niger B0201,in order to obtain mutant strains with enhanced ability to produce tannase. The optimal treatment condition were determined,and dilution pate culture method was used to screen mutant strains.The color changing circle method and spectrophotometry was used to analyze tannase production,and the best mutation time was 180 s,and the positive mutation rate was 19%. The high concentration of2% tannic acid was utilized for color plate screening 850 strains.90 strains of bacteria were screened by liquid shake flask fermentation,and the strain of extraordinary yield of B1401 was achieved.After the mutation,the enzyme activity was 17.61 U / g,which was about double as much as that of the original strain 8.94 U / g,and the stability of the enzyme production was good.