Research of enzymolysis technology of the British red kidney bean protein antioxidant peptide

Study of enzymolysis technology of kidney bean protein antioxidative peptide with the British red kidney beans as raw material.First of all,the degree of hydrolysis and total antioxidant capacity as evaluation index for screening of enzyme.alkaline protease was selected from six different enzymes by single factor and orthogonal design.The results showed that the kidney bean protein could be effective hydrolyzed by the alkaline protease and the highest antioxidant capacity of enzymolysis products.The optimal conditions were substrate concentration of 5.0%( w/v),enzyme concentration of 4000 u/mL,pH10,temperature of 55 ℃,hydrolyzing time of 2 h,under this condition of the total antioxidant capacity to 186.97 U/mgpro. British red kidney beans peptide showed strong antioxidant activity.