Extraction and antibacterial activity of procyanidins from Aronia melanocarpa

Response surface methodology was employed to optimize the extraction conditions of procyanidins from Aronia melanocarpa and antibacterial activity was researched by Oxford- cup tests. Results indicated that the optimum extractive conditions were determined as follows: ethanol concentration 62%,liquid to solid ratio 25 ∶ 1 m L / g,extraction temperature72 ℃,extraction time 60 min,the content of procyanidins through this optimized procedure was 3.32%. The content of procyanidins were four times as much purification as crude extracts after the treatment by AB- 8 macroporous resin,which content of procyanidins was 14.29%.Bacteriostatic experiment of procyanidins from black chokeberry on several strains tested showed antimicrobial effect as follows: Bacillus subtilis > Staphylococcus aureus > Escherichia coli > Saccharomyces cerevisiae.Antibacterial activity increased with increasing of procyanidins concentration and crude extracts of Aronia melanocarpa procyanidins had better antibacterial activity.