Purification of flavonoids from wheat germ by macroporous resin

Based on the previous study of the extraction of flavonoids from wheat germ( FWG),the purification of FWG was performed by macroporous resin.Seven kinds of macroporous resin were selected to compare their purification ability by using adsorption and desorption rate as the indicators.Furthermore,the best one was used to optimize the separation process.The data indicated that H103 had better adsorption and desorption property for purification. The optimal adsorption parameters were as follows: the sample concentrations was about 0.65 mg / m L,the flow rate was 2.0 BV / h,the desorption solution concentration was70% ethanol,the desorption rate was 2.0 BV / h.After one run treatment with H103 resin,the extraction of FWG was increased12.26 times,and the content of flavonoids was up to 11.77% from 0.96%.