Analysis of volatile compounds in Dunaliella Salina with HS-SPME-GC-MS method

The volatile components in Dunaliella Salina were determined by headspace solid phase microextraction( SPME) and gas chromatography-mass spectrum( GC-MS).The result showed that 47 volatile compounds in Dunaliella Salina were isolated and identified.Alcohols,ketones,aldehydes and carboxylic acid were the primary contents,representing 93.69% of the total volatile compounds as described. However,the fragrance components were mainly composed of the productions of β-carotene metabolism or degradation,such as β-cyclocitral,β-ionone,α-ionone and 3,5,5-Trimethyl-3-cyclohexene-1-one etc.Furthermore,the fishy odor compounds primarily included 2-camphanol,trans-bicyclol4.4.0decan-1-ol-3-one,4-2,2,6-trimethyl-7-oxa-bicyclo4.1.0hept-1-yl-3-butene-2-one,isophorone etc. The pungent odor compounds primarily included furan-3-carboxylcacid-5-tert-butyl-2-p-tert-butyl-phenoxy methyl,5-isopropyl-2-methyl phenol as well as 2,6-diethyl-pyrazine etc.Overall,the fragrance,fishy and pungent odor were the main flavor in Dunaliella Salina.