Optimization of extraction of polysaccharides from Osmunde japonica leaves by response surface methodology and their inhibitory effects on tumor

The study was to explore the optimum processes of extraction polysaccharides from Osmunda japonica leaves and the effect of Polysaccharides on cancer cells. Using the ultrasonic microwave synergistic extraction,solid- liquid ratio,microwave power and extraction time as factors,based on the single factor experiment,the response surface methodology( RSM) Box- Benhnken center combination experimental design was employed to optimize the extraction of polysaccharides of Osmunda japonica.The results of optimum extraction conditions were as follow: the solid- liquid ratio was 1∶ 35( g / m L),microwave power 353 W,extraction time was 250 s.Under these conditions,the extraction yield was 0.236%. According to the Cytotoxicity test,the inhibitory effect of polysaccharides from Osmunda japonica on the Hep G-2 cancer cells had been studied.The results of cytotoxicity test indicated that the concentration of polysaccharides were 0.001 mg / m L,10 mg / m L separately,the inhibition rate was 18.13%,72.65% respectively.The vaule of IC50 was 0.474 mg / m L.