Study on extraction technology of Polypeptide from Phaseolus Vulgaris

In this paper,taking Phaseolus Vulgaris as row material,alkaline protease hydrolysis was used to prepare coloured kidney bean polypeptide.Enzymolysis conditions such as enzymolysis temperature,enzymolysis time,p H value and enzyme dosage were optimized by means of designing orthogonal experiments.The results showed that the optimum conditions were as follows: temperature 60 ℃,enzymolysis time 4 h,p H7.5,enzyme dosage 2000 U /g. Under this condition,the concentration of polypeptides in Phaseolus Vulgaris was 0.954 mg / m L. This paper proposes a method for extracting polypeptides from Phaseolus Vulgaris,and aims to lay a foundation for further exploration and utilization of polypeptides from Phaseolus Vulgaris in future.