Optimization of ultrasonic-assisted extraction of total saponin from Acanthopanax sessiliflorus using response surface methodology

The root stem of Acanthopanax sessiliflorus( Rupr. et Maxim.) Seem was used as the raw material,ultrasonic assisted extraction of total saponins from Acanthopanax sessiliflorus. The effect of material- to- liquid ratio,ethanol concentration,ultrasonic time,ultrasonic power on the extraction result of the total saponin of Acanthopanax sessiliflorus was studied. On the basis of single factor experiment,Box- Behnken Design was applied to build a regression model,with the yield rate of total saponin as response value. Establish regression model and according to this,the optimal extraction combination was follows: material- to- liquid ratio 1 ∶ 50,ethanol concentration 68%,ultrasonic time 41 min,ultrasonic power 350 W. In this condition,the yield of the total saponin was 1.577%.The true value of the experiment accords with the predictive value of the model was 1.586%,show that response surface center combination method is reasonable and feasible,which can optimize the extraction process of total saponins from Acanthopanax sessiliflorus.