Optimization of enzymatic extraction of polysaccharides from Ganoderma lucidum using response surface methodology

Effects of different kinds of enzymes on the extraction of Ganoderma lucidum polysaccharides have been investigated,with the crude polysaccharides yield from Ganoderma lucidum fruit body as the index. The results showed that four enzymes,i.e.,papain,lyticase,cellulase,and pectinase,could increase the polysaccharides yield.The optimal combination and dosages of the four enzymes were determined by the single factor experiment method and the response surface methodology,which was papain 1.6%,lyticase 2.1%,cellulase 1.6%,and pectinase 2.3%,respectively. Then the response surface methodology was used again to determine the optimal enzymatic extraction conditions,with the temperature,time,p H value,and liquid to solid ratio as the variables,as well as the crude polysaccharides yield as the response. The optimal enzymatic extraction conditions were as follow: temperature 60 ℃,time 118 min,p H value 4.6,the liquid to solid ratio of 16 ∶ 1. Under these conditions,the crude polysaccharide yield was 4.41%,which was 41.3% higher than that of 3.12% extracted by traditional hot water extract method.