Enzyme hydrolysis preparation and ACE inhibitory activity of bioactive peptides from Meretrix lusoria

Meretrix lusoria protein was hydrolyzed by protease enzyme through the ternary quadratic orthogonal design based on the single factor experiment,and bioactivity against angiotensin converting enzyme( ACE) of peptides was investigated. Optimal hydrolysis conditions were found that hydrolysis time was 3 h,the substrate concentration was 10%,the value of p H was 8.7,the addition of enzyme was 3% and the hydrolysis temperature was 51 ℃. The degree of hydrolysis was 33%. In vitro ACE inhibitory activity of bioactive peptides from Meretrix lusoria was performed by high performance liquid chromatography.The results suggested that the activity against the ACE of bioactive peptides was 35%.