Isolation,purification and identification of protein with cholesterolreducing activity from the supernatant of Lactobacillus reuteri

CHEN Chen; YU Rui-li

doi:10.13386/j.issn1002-0306.2016.22.031

CHEN Chen, YU Rui-li. Isolation,purification and identification of protein with cholesterolreducing activity from the supernatant of Lactobacillus reuteri[J]. Science and Technology of Food Industry, 2016, (22): 200-204. DOI: 10.13386/j.issn1002-0306.2016.22.031

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Isolation,purification and identification of protein with cholesterolreducing activity from the supernatant of Lactobacillus reuteri

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The cell- free supernatant( CFS) of Lactobacillus reuteri DSM122460 exhibited the cholesterol removal ability and the effective components were predicted as proteins. The active component was purified by ultrafiltration,ammonium sulfate precipitation,and DEAE- Sepharose F.F anion exchange chromatography. The specific activity of purified component was 61.6 U / mg with a purification factor of 32.4. The purified protein was electrophoresis pure determined by SDS- PAGE,and the estimated molecular weight was about 60 ku. The active protein was identified as a hypothetical protein by mass spectrometry and its function remains to be further studied.

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Science and Technology of Food Industry

Isolation,purification and identification of protein with cholesterolreducing activity from the supernatant of Lactobacillus reuteri

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