Study on fluorescence spectra mechanism of vitamin C

Taken standard vitamin C for research objects,fluorescence emission spectra were measured with fluorescence spectrophotometer and the excitation wavelength were 280 ~ 350 nm. The results showed that the fluorescence excitation and emission wavelengths of the vitamin C solution were 310 nm and 400 nm. The second order derivation of fluorescence spectrum had four extreme points and three inflection points which were 328,345,355,372,395,425,525 nm.Based on the theory analysis,we come to the conclusion that the fluorescence peak at395 nm was formed by- OH n→σ*- electron jumps.The dienes alcohols structure emitted a π→π*- electron jumps of maximum peak that were a little red shift at 425 nm and 525 nm.The reason was strong affected by conjugated system.Each of molecule fluorescent group were restricted and superimposed.The molecular clusters and H bond of water molecule made maximum fluorescence peak of vitamin C solution at 400 nm.