High-performance liquid chromatography method for quantitative determination of curcuminoids in rhizomes of Curcuma Longa L.and curcumin beverage

A HPLC method was established for the determination of curcuminoids content in rhizomes of Curcuma Longa L. and curcumin beverage.The samples were extracted in ethanol by ultrasonic treatment.The determination by RP-HPLC was carried out using DIKMA Platisil ODS column( 4.6 mm × 250 mm,5 μm) and acetonitrile-water( p H = 3.0 adjusted by phosphoric acid) with gradient elution at a flow rate of 1 m L/min. The UV detection wavelength was 425 nm and the column temperature was set at 30 ℃.The calibration curves were linear in the range of 1.00 ~ 200.00,0.96 ~ 192.00,0.96 ~ 192.00 mg / L( R2> 0.999) for curcumin,demethoxycurcumin and bisdemethoxycurcumin.The average recoveries were 106.87%( RSD = 1.24%),105.28%( RSD = 0.48%) and 96.86%( RSD = 0.26%),respectively. The method was simple,rapid and reproducible,and can be used for the determination of curcuminoids in rhizomes of Curcuma longa L.and curcumin beverage.