Optimization of preparation of chitosan / walnut protein complex film by response surface methodology

To study the preparation conditions of chitosan / walnut protein complex film,the preparation conditions were optimized through response surface methodology.With ratio of chitosan and walnut protein,glycerol addition,p H,glutanaldehyde addition being response factors,the tensile strength,elongation and water vapor permeability of edible film were measured and the quadratic regression models of indicators were achieved.The results showed that the optimal preparation conditions were ratio of chitosan and walnut protein 0.50,glycerol addition 2.00 g,p H value 3.23,glutanaldehyde addition 0.10 g.Under this condition,the actual properties of edible films were( 18.985 ±1.102) Mpa,72.79% ± 2.172%,( 4.41 ± 0.125) g·mm /( m2·d·k Pa).