Research on dual-enzymatic extraction of polysaccharides from Douchi

Based on the single factor experiments,the orthogonal experimental design was employed to optimize the extraction conditions of polysaccharide from douchi via dual-enzymatic.The results showed that the optimum dual-enzymatic extracting conditions were as follows: firstly,the ratio of water to raw material 1∶ 20( g ∶ m L),p H7.0and extraction temperature 50 ℃,enzyme dosage of papain 2% and hydrolysis time 150 min.Secondly,p H7.0 and extraction temperature 55 ℃,with flavourzyme 2.5%,hydrolysis time 150 min.Under these step by step enzymatic hydrolysis conditions,the extraction yield was 15.90%. The optimization of extraction process technology of polysaccharides from douchi was reasonable and feasible and yield was higher.