Extraction and analysis of monosaccharide compositions of wild Ramaria sp.polysaccharide

To optimize the extraction conditions for the polysaccharides from Ramaria sp.,based on the single factor trials,the response surface methodology were applied. Using the high performance liquid chromatography for the determination of the monosaccharide composition.The results showed that the optimum extraction conditions was as follows: temperature 91 ℃,water-to-material-ratio 32 ∶ 1( m L / g),time 3.2 h.Under these conditions,the actual extaction rate was 1.97%. The PMP-HPLC results showed that the Ramaria sp. polysaccharides was composed of mannose,ribose,glucose,galatose,xylose,and fucose and their molar ratio was about 45.8∶ 10.6∶ 76.4∶ 8.53∶ 4.63∶ 5.24.Conclusion: The RSM method could be used for optimizing the extraction condition of Ramaria sp.polysaccharides and the PMP-HPLC method could be used for determination of polysaccharide compositions.