Optimization of decoloring process of enzymatic hydrolysate of fish roes peptide by response surface methodology

Enzymatic hydrolysate of fish roes peptide was decolored by activated carbon.The effect of the dose of activated carbon,time,temperature and p H on the decoloring was investigated by decoloring rate and peptide retention rate detected.Based on the results of single factor experiments,the decoloring process was optimized by response surface methodology. The results showed that the optimal conditions were as follows: the amount of activated carbon 10.5%( m / m),time 41 min,temperature 55 ℃,p H2.8.Under this optimal conditions,the decoloring rate was 77.51% ± 1.49%,the peptide retention rate was 82.26% ± 2.82% which was not significant with the theoretical value.The decoloring process was simple,reliable,high peptide retention rate and high decoloring rate.The results would provide a theoretical foundation for the development of fish roes peptide.