Optimization of culture medium and fermentation conditions for neutral protease produced by Bacillus coagulans Liu-g

ZHANG Ying-ying; GUO Qian; XIONG Li-xia; ZHANG Hong-xing; XIE Yuan-hong; LIU Hui; LIANG Xin-bei; LIAN Zheng-xing

doi:10.13386/j.issn1002-0306.2016.21.021

ZHANG Ying-ying, GUO Qian, XIONG Li-xia, ZHANG Hong-xing, XIE Yuan-hong, LIU Hui, LIANG Xin-bei, LIAN Zheng-xing. Optimization of culture medium and fermentation conditions for neutral protease produced by Bacillus coagulans Liu-g[J]. Science and Technology of Food Industry, 2016, (21): 150-154. DOI: 10.13386/j.issn1002-0306.2016.21.021

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Optimization of culture medium and fermentation conditions for neutral protease produced by Bacillus coagulans Liu-g

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In order to improve the activity of neutral protease produced by Bacillus coagulans Liu-g1,single factor test and orthogonal test on Bacillus coagulans Liu-g1 protease activity were carried out. The results showed that the optimal culture medium formula was 1% of soybean powder,4% of corn starch and 0.6% of calcium carbonate.The results also indicated that the best initial p H was 7.5,fermentation volume of 30 m L /250 m L,incubation temperature at 45 ℃,fermentation time for 72 h. The protease activity reached 243.874 U / m L in such conditions,5.8 times the amount of initial conditions.

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Optimization of culture medium and fermentation conditions for neutral protease produced by Bacillus coagulans Liu-g

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