Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

Objective: To study the interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA. Methods: The interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA was investigated by fluorescence spectroscopy,viscosity measurements and thermal dissolution assay in the Tris- HCl buffer( p H7.4) at 293 K and 310 K,Acridine Orange( AO) as the fluorescent probe.Results: The fluorescence quenching of Ginkgol C15 ∶ 1 by AO- DNA was a static quenching procedure. The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicated the interaction between Ginkgol C15 ∶ 1 and AO- DNA was intercalation. Conclusions:The results of viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15∶ 1 and AO- DNA.