Study on condition optimizing of extraction technique of squid ink polysaccharide from Sepia pharaonis by response surface methodology

To explore the optimum extraction conditions of squid ink polysaccharides from Sepia pharaonis squid ink,this study was conducted to provide a theoretical basis for the development and utilization of squid ink. By single factor tests,enzyme dosage,solid- liquid ratio,reaction temperature,reaction duration and p H were investigated.And on the basis of single factor tests,according to the( Box- Behnken) experimental design protocol,enzyme dosage,solid- liquid- ratio,reaction temperature,and p H were selected to the four factors and the response surface experiment was carried out by response surface analysis. The results showed that their optimal conditions were defined as follows: enzyme amount 46 mg / g,solid- liquid- ratio 1∶ 19.7,reaction temperature 54.1 ℃and p H9.0. Under this condition,the polysaccharide yield was as high as 2.140%.