Compared study on extraction of total gypenosides by different methods

To study the extraction effects of traditional reflux techniques,enzyme techniques,microbial fermentation techniques,the content,consititude,antioxidant activities of total gypenosides were evaluated.The results exhibited that the total gypenosides contents by complex enzyme method were reached to 7.69%,Which increased by 31%comparing with traditional method,but transformation of saponins constituents did not detected signiflcantly by the enzyme techniques. The wall- breaking extraction effects of fermentation method was most excellent,the total gypenosides contents by this method were reached to 8.34%,Which increased by 42.1% comparing with traditional method,and transformation of component saponins involved in the fermentation process,such as the contents of gypenosides V- AH( ginsenoside Rg3) were increased. Due to fermentation,IC50 of DPPH radical scavenging capacity was reduced from 1.544 to 0.562 mg / m L comparing with traditional method,and ascorbic acid equivalent antioxidant capacity on reducing power of Fe³⁺ were reached to( 236.299 ± 12.785) mg V_C/ g solids.In summary,the microbial fermentation extraction method was prior to other methods.