Cloning,identification and bioinformatics analysis of β-fructosyltransferase from Aspergillus oryzae A-F04

Based on the efficient expressing Aspergillus oryzae strain A- F04,the DNA and RNA of strain A- F04 were extracted,PCR and RT- PCR strategies were used to obtain β- FTase sequences,thus,β- FTase gene bank was constructed. Evolutionary tree was built,and physicochemical property including formula weight,isoelectric point and amino acid composition were predicted,the possible glycosylation sites were predicted.SWISS MODEL tool was used to predict β- FTase tertiary structure. Experimental results showed that β- FTase gene was successfully identified in strain A- F04. Strain A- F04 's coding region was 1630 bp,including an intron,between173 bp and 224 bp,CDS were 1578 bp. Bioinformatics analysis program analysis showed that β- FTase was hydrophilic stable protein,its formula weight was 57.4653 ku,isoelectric point was 4.83,composed by 525 amino acids,within 6 possible glycosylation sites. Signal P program showed that the amino acids at 1 to 17 sites were signal peptide.The tertiary structure model constructed by SWISS- MODEL contributes to the β- FTase's structure and function exploration.