Optimization of fermentation and expression conditions for production of marine lipase by Bohai sea-9145

RAN Fan-jiao; SUN Mi; BAO Jing; HAO Jian-hua

doi:10.13386/j.issn1002-0306.2016.16.027

RAN Fan-jiao, SUN Mi, BAO Jing, HAO Jian-hua. Optimization of fermentation and expression conditions for production of marine lipase by Bohai sea-9145[J]. Science and Technology of Food Industry, 2016, (16): 178-183. DOI: 10.13386/j.issn1002-0306.2016.16.027

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Optimization of fermentation and expression conditions for production of marine lipase by Bohai sea-9145

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To effectively improve the production of the Bohai sea- 9145 lipase,single factor experiment,PB experiment and BB surface response experiment were used to optimize the fermentation conditions of the recombinant strain Bohai sea-9145.The optimal conditions were as follows: the initial p H value of the medium was8.0,the inoculation amount was 1.5%,and after 3.5 hours of incubation,the expression of the lipase was induced by adding IPTG to a final concentration of 0.5 mmol / L at 16 ℃ for 15 hours with the shaking speed of 200 r / min.After optimization,the final activity of the recombinant lipase was increased by 9.72 times.

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Science and Technology of Food Industry

Optimization of fermentation and expression conditions for production of marine lipase by Bohai sea-9145

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