Development and evaluation of duplex pcr assays for detection of Pseudomonas fluorescens in foods

GAO Lin; CHEN Wan-yi; XU Yu; LIU Zhen-min; YOU Chun-ping

doi:10.13386/j.issn1002-0306.2016.15.048

GAO Lin, CHEN Wan-yi, XU Yu, LIU Zhen-min, YOU Chun-ping. Development and evaluation of duplex pcr assays for detection of Pseudomonas fluorescens in foods[J]. Science and Technology of Food Industry, 2016, (15): 294-299. DOI: 10.13386/j.issn1002-0306.2016.15.048

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Development and evaluation of duplex pcr assays for detection of Pseudomonas fluorescens in foods

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Duplex PCR assays were developed for the detection of Pseudomonas fluorescens,based on its gyr B gene and apr gene. Among all the bacterial strains used in this research,duplex PCR amplified two specific products( 384 bp and 194 bp) from the strains of Pseudomonas fluorescens,but not from the negatives. The specifity of our assays was 16.9 fg / μL for pure DNA and 1.8 CFU / m L for UHT milk( 250 m L) with 24 h enrichment.So,the methods described in our study can be adopted to detect Pseudomonas fluorescens in food samples with higher sensitivity and specificity.

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Development and evaluation of duplex pcr assays for detection of Pseudomonas fluorescens in foods

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