Optimization of extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology

The ultrasound- assisted extraction of flavone from the Pinus massoniana pollen was carried out. The effects of ethanol concentration,extraction time,material- liquid ratio and extraction numbers on the extraction rate of flavone were studied by single factor experiment,and the extraction process of flavone was optimized by the central composite design- response surface methodology. The results showed that,the optimal extraction conditions of flavone from the Pinus massoniana pollen were 62.5% of ethanol concentration,28.8 minutes of extraction time,1 ∶ 24 of material- liquid ratio and 3 times of extraction number. Under this optimal condition,the actual extraction rate of flavone reached 1.37%,which was close to the theoretic value.It was feasible to optimize the extraction process of flavone from the Pinus massoniana pollen by using central composite design- response surface methodology.