Screening and identification of a strain producing polyhydroxybutyrate

ZHANG Yong- jie; DONG Fei-fei; DENG Zhi-wei; ZHANG Ting-ting; YANG Sheng-yu

doi:10.13386/j.issn1002-0306.2016.14.037

ZHANG Yong- jie, DONG Fei-fei, DENG Zhi-wei, ZHANG Ting-ting, YANG Sheng-yu. Screening and identification of a strain producing polyhydroxybutyrate[J]. Science and Technology of Food Industry, 2016, (14): 223-226. DOI: 10.13386/j.issn1002-0306.2016.14.037

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Screening and identification of a strain producing polyhydroxybutyrate

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A polyhydroxybutyrate( PHB) strain was isolated from sewage by Nile blue fluorescence staining combining with Sudan black B,which was based on glycerol as the carbon source. A series of analyses on physiological and biochemical characterization and sequence analysis of 16 S rRNA revealed that it belonged to the genus Pseudomonas pudita,which named Pseudomonas pudita PP25.PHB was extracted with sodium hypochlorite- chloroform.Finally,a high yield PHB strain named PP25 was determined by UV spectroscopy,Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy,yield of PHB was 2.456 g / L.

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Screening and identification of a strain producing polyhydroxybutyrate

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