Determination of chlorogenic acid and protocatechuic acid in potatoes by HPLC

Objective: To establish a method of detecting the content of chlorogenic acid and protocatechuic acid in potato tuber by High Performance Liquid Chromatography( HPLC),this method was used to determine the change of chlorogenic acid,protocatechuic acid content in two species potatoes during storage.Methods: Gradient elution HPLC was used to determine chlorogenic acid and protocatechuic acid.The samples were separated by reversed- phase C18 chromatographic column using 1% formic acid and 100% methanol as a mixed mobile phase. The detection wavelength was set at 326 nm and 280 nm respectively.The column temperature was set 40 ℃. Results:The calibration curves showed good linear relationship between the peak areas and concentrations was50~500 μg / m L for chlorogenic acid,200 ~ 1000 μg / m L for protocatechuic acid,the related coefficient was 0.9999,0.9992. The RSD of precision were all lower than 0.35%.The RSD of stability were all lower than 1.68%.The RSD of repeatability was 2.07% for chlorogenic acid,3.58% for protocatechuic acid. Standard addition recovery of chlorogenic acid,protocatechuic acid was 98.29%,97.81% with relative standard deviation( RSD) of 1.46%,1.31%( n = 5).Conclusion: The method was rapid,accurate with wind linear range and good separation results,which was suitable for the analysis of chlorogenic acid,protocatechuic acid in potato tuber.