Surface- Enhanced Raman Spectroscopy( SERS) assay method for pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride in health care products

Objective: A fast assay method for pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride was developed.Method: A Surface- Enhanced Raman Spectroscopy( SERS) assay method was used to detect the pioglitazone hydrochloride,rosiglitazone hydrochloride and phenformin hydrochloride. Result: The concentration of the SERS method for pioglitazone hydrochloride and rosiglitazone hydrochloride was 4 μg / m L,and for phenformin hydrochloride was 1 μg / m L. The SERS spectrums under three different p H conditions were studied,and the results showed that the p H of the system had obvious effect on the SERS,and the SERS was most enhanced when the p H was 2.0. For detecting health care products,the concentration of the SERS method for pioglitazone hydrochloride and rosiglitazone hydrochloride was 200 μg / m L,and for phenformin hydrochloride was100 μg / m L.The SERS method was simple and rapid and could be finished in fifteen minutes.Conclusion: The SERS method could be a convenient tool for field detection of health care products.