Characterization of a neutral protease promoter derived from a Bacillus amyloliquefaciens strain

To obtain the DNA elements responsible for transcriptional regulation of the gene expressing 1398 neutral protease( npr E),the genome sequencing of the native bacillus strain was performed and analyzed,the complete operon including upstream region of the possible location of 1398 npr E cassette was introduced into B.subtilis for heterologous expression.gfp was then used as the reporter gene for full investigation of the promoter of 1398 npr E( P1398) and was compared with known promoter P_xyl Aand P43. It was found that,P1398 was an efficient and substrate- induced promoter as well as a key factor in the expression of 1398 Npr E.Stable expression of 1398 Npr E in B.subtilis was detected and the highest enzyme activity was 1210 U / m L in 164.Compared with P_xylAand P43,the advantage of P1398 was its capability of bringing better yield of GFP protein in B. subtilis with medium used for industrial production of enzymes,the GFP level was 214% of that mediated by P_xyl A,therefore,P1398 was a good candidate of promoter choice for construction of recombinant enzymes' production using Bacillus.