Vector construction and biotransformation of double subunits salvianolic acid- esterase

In order to achieve the salvianolic acid- esterase express in vitro,the salvianolic acid- esterase were heterologous expressed by E.coli and Pichia pasioris respectively.The E.coil system constructed expression vector with p ET 28 a and p ET 32 a and carried out induced expression and coexpression of the recombinant E.coil. The Pichia pastoris system constructed coexpression vector with p PIC9 K and carried out induced expression of the recombinant Pichia pastoris. The research found that the salvianolic acid- esterase were induced in E.coli and Pichia pasioris.The protein was highly expressed in E.coli system,but it did not exhibit the enzyme activity. The salvianolic acid- esterase were expressed in Pichia pasioris,and the expression product had salvianolic acid-esterase activity.But the quantity of expression was not high.It showed that the E.coli expression system was more suitable for the salvianolic acid- esterase and provided a exogenous expression method of salvianolic acid-esterase.