Solubility evaluation of Perilla frutescens seed oil in supercritical carbon dioxide and its antioxidative activity

In this research,oil was extracted from Perilla frutescens seed by using supercritical carbon dioxide( SC-CO₂) to investigate the effect of pressure and temperature on the solubility and yield.The solubility data was correlated based on the Chrastil model,fatty acid compositions of Perilla frutescens seed oil was further investigated through gas chromatography- mass spectrometry.Resualts showed that solubility was increased with increasing pressure at constant temperature and decreased with increasing temperature at constant pressure.The highest solubility was obtained at 313 K,35 MPa.A solubility model of Perilla frutescens seed oil based on the temperature and the density of CO₂ was obtained. Morever,GC- MS analysis results showed no significant difference in the fatty acid composition of Perilla frutescens seed oil among different extraction methods and over90% of poly unsaturated fatty acid was obtained from Perilla frutescens seed oil.DPPH- scavenging capacity study showed that the IC₅₀ value of oil extracted using SC- CO₂ and Soxhlet method were 22.66 mg / m L and27.76 mg / m L.