Optimal enzymatic preparation conditions and antioxidative activity of giant salamander bioactive peptide

In this paper,enzymatic preparation conditions of giant salamander bioactive peptide and its physicochemical properties were studied. Based on the degree of hydrolysis,the preparation method was optimized by response surface methodology. The optimal temperature,the reaction time,the ratio of liquor to material,enzyme amounts,and p H were 50 ℃,1.5 h,2 ∶ 1( m L / g),70 U / g,and p H8.91,respectively,the highest degree of hydrolysis was obtained,up to 19.61% ± 0.12%. Protein content of the bioactive peptide was 52.40% ±1.28% and the molecule weight of the most bioactive peptide was below 1000 u. At the concentration of 30 mg / m L,the scavenging rates of giant salamander bioactive peptide against hydroxyl radical and superoxide anion were81.83% and 56.27%,respectively.