Content determination of polyphenol from Curcumin cymlin L. using response surface method

The polyphenol from Xinjiang Curcumin cymlin L. was abstracted by ultrasonic-assisted extraction,and the extraction yield was measured by Lowry method with Folin phenol.According to single-factor test,the extraction yield was determined by the method of response surface analysis( RSA) with four factors( the ethanol concentration,solid/liquid ratio,ultrasonic time and ultrasonic temperature),And the notability difference was analyzed with the statistic method of ANOVA. Results showed that the optimal conditions were as follows: ethanol concentration 70%,solid/liquid ratio1 ∶ 20,ultrasonic time 60 min,ultrasonic temperature60 ℃.Under such conditions,the actual amount of polyphenols extracted for 13.8 mg/g,with a difference of 4.5% compared with predictive value,this method of extracting higher than 139% with reflux extraction.Optimization of Using Response Surface Methodology can greatly improve the content determination of polyphenols from Xinjiang Curcumin cymlin L.