Cloning and sequence analysis of wild ginseng phospholipid hydroperoxide glutathione peroxidase cDNA

Phospholipid hydroperoxide glutathione peroxidase( PHGPx) has significant antioxidant activity,avoiding the harm by peroxidation in the cell membrane.To clone the full-length c DNA encoding PHGPx gene from Panax ginseng,the RACE technology was used in this study. The sequence analysis showed that: the full length of the cloned fragment was 490 bp.It encoded 162 amino acids and contained 3 sub-conservative domains which were specific in eukaryonic phospholipid hydroperodixe glutathione peroxidase.The encoding protein was a stable nontransmembrane hudrophilic protein. The sequence of amino acids was similar with PHGPx of citrus and castor,homologous rates of amino acids were 76% and 75%. And then,the phylogenetic tree of nine plants was constructed.