Gene expression of the bile salt hydrolase in Lactobacillus plantarum ST- Ⅲ

Bile salt hydrolase( BSH),the enzyme deconjugating bile potentially plays an important role in reduction of blood cholesterol level,which has received a widespread attention. In this study,the recombinant Lactobacillus plantarum ST-Ⅲ were constructed by overexpressing four BSH genes and the BSH enzyme activities were determined.The result showed that L.plantarum ST-Ⅲ harboring BSH1 had a higher enzyme activity than the other three strains,then was the strains harboring BSH3 and BSH2,and the lowest activity was shown in ST-Ⅲoverexpressing BSH4,which indicated that the BSH1 might play a more important role in the hydrolysis of the conjuncted bile salts,then was BSH3,BSH2 and BSH4 successively.