Effect of blueberry leaf polyphenols on free radicals in the formation of formaldehyde in squid supernatant

The free radical which is related to formaldehyde in squid supernatant was determined by ESR. At the same time,the effect of blueberry leaf polyphenols on free radicals in squid supernatant was studied at high temperature.The results showed that Fe²⁺ promoted( CH₃)_3N· in squid supernatant.Different concentrations of blueberry leaf polyphenols decreased( CH₃)_3N·in the squid supernatant,and the higher concentration decreased more significantly.The( CH₃)_3N· was increased with heating time prolonging. Scavenging capacity of blueberry leaf polyphenols was decreased when heating time was over 75 min. The( CH₃)_3N· were enhanced with the temperature increasing. There was no affect on blueberry leaf polyphenols scavenging( CH₃)_3N· at high temperature heating for 15 min.The( CH₃)_3N· in reaction system of p H6.0 was more than that in reaction system of p H7.0.The scavenging ability in p H6.0 was stronger than in p H7.0. This work indicated that radical reaction existed in non-enzymic formation mechanism of formaldehyde in squid supernatant. The formation of formaldehyde was controlled through the reaction of blueberry leaf polyphenols with free radical.