Activation effects of extracellular polysaccharides produced by Pleurotus ferulae Lanzi on murine macrophage

The culture system of macrophages was established to investigate the activation effects of extracellular polysaccharides produced by Pleurotus ferulae Lanzi( IPP) on murine macrophage. Murine macrophages were cultured in sterile environment. Phagocytic activity,the content of nitric oxide synthetase( NOS) and nitric oxide( NO),the levels of tumor necrosis factor-α( TNF-α),interleukin-1( IL-1),and interleukin-12( IL-12) in culture supernatant were examined with neutral red phagocytosis test,fluorescein labeled probe technique,griess reaction and enzyme- linked immunosorbent assay( ELISA),respectively. The results showed that IPP in the range of 25 ~200 mg / L reduced absorbance of neutral red in cytoplasm( p < 0.05),improved the secretion level of IL- 1( p <0.05) and IL- 12( p < 0.05) in culture supernatant. And IPP in the range of 50 ~ 200 mg / L increased the content of NOS( p < 0.05) and NO( p < 0.05),and the secretion level TNF-α( p < 0.05). So murine macrophages cultured in vitro could be activated by IPP at a certain concentration.